ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Post-thaw Asian Elephant Spermatozoa: The Effect of Equilibration Time on Progressive Motility, Viability and Acrosome lntegrity)

สื่อสิ่งพิมพ์:pdf

Abstract

The objective of this study was to investigate the effect of equilibration times on progressive motility and acrosome integrity of frozen-thawed Asian elephant spermatozoa. Semen samples were collected from 2 elephant bulls (EM 1 and EM 2;4 ejaculates per bull) by manual manipulation. Semen were diluted with TEST containing 5% glycerol final concentration frozen in liquid nitrogen after 0,1,2,3,4 and 5 hours of equilibration times. The frozen semen were thawed and evaluated for progressive motility, viability and acrosome integrity. In EM 1, the percentage of progressive motility of frozen-thawed spermatozoa after equilibrated for 0,1,2 and 3 hours did not differ from those found after equilibration for 4 and 5 hours. In EM2, the percentage of progressive motility of frozen-thawed spermatozoa after 0,1,2 and 3 hours. In EM 2, the percentage of progressive motility of frozen-thawed spermatozoa after 0,1,2 and 3 hour equilibrations were significantly higher (p<0.05) than those after 4 and 5 hour eqilibrations. The different equilibration time (o-5h) did not effect on viability and acrosome integrity of frozen-thawed spermatozoa. This finding indicates that the equilibration times prior freezing affected to frozen-thawed sperm motility with the trend that higher motie spermatozoa were obtained after 0-3 hour equilibrations. The obtained knowledge will be aided in the development of cryopreservation technique and artificial insemination in Asian elephants in the future.

Article Info

วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 019, Issue 2, May 09 - Aug 09, Page 80 - 89 |  PDF |  Page